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Horizontal High Pressure Autoclave

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Horizontal High Pressure Autoclave

Introduction to Horizontal High Pressure Autoclave

A Horizontal High Pressure Autoclave is an advanced steam sterilization system engineered for bulk loads, frequent cycles, and stringent hospital/pharma standards. Using saturated steam at 121–134°C under elevated pressure (typically 15–30 psi), it achieves rapid, uniform, and validated sterilization of instruments, glassware, rubber stoppers, culture media, and dressings. With a rugged horizontal chamber, jacket heating, and precision control, it is ideal for CSSD rooms, microbiology labs, cleanrooms, and vaccine & biotech facilities. Built with GMP-ready documentation and safety interlocks, it ensures consistent results and compliance while protecting heat-resistant devices and operators.


Working Principle and Process Flow for Horizontal High Pressure Autoclave

The autoclave relies on saturated steam under pressure to denature proteins and destroy all microbial life, including spores. A typical cycle includes: (1) Preheating/jacket heating, (2) Air removal via gravity displacement or pre-vacuum pulses for uniform steam penetration, (3) Exposure—holding the set temperature/pressure for the validated time, (4) Exhaust—controlled depressurization, and (5) Drying—vacuum with jacket heat to remove residual moisture. Automated PLC/HMI controls monitor temperature, pressure, and time, while safety valves, door interlocks, and over-temperature protection ensure reliable and safe operation for every load.

Types of Horizontal High Pressure Autoclave

  • Horizontal Cylindrical Autoclave – compact footprint for sterilizing instrument sets, media, and glassware in hospitals and labs.
  • Horizontal Rectangular Autoclave – wide door and trolley loading for large packs, linen, and bulk loads in CSSD and pharma.
  • Triple-walled Jacketed Autoclave – chamber, jacket, and insulation for faster heat-up, uniformity, and energy efficiency.
  • Pre-Vacuum (Class B) Horizontal Autoclave – vacuum-assisted air removal for complex, porous loads and wrapped instruments.
  • PLC/HMI Fully Automatic Autoclave – recipe management, cycle printouts, alarms, and data logging for validation and traceability.

Key Features and Specifications

  • Operating Range: 121–134°C with 15–30 psi; multi-stage programmable cycles for liquids, instruments, and textiles.
  • Construction: SS304/SS316L chamber with radial/hinged door locking, high-grade gaskets, and robust frame for 24×7 duty.
  • Controls & Safety: PLC/PID control, digital sensors, door interlocks, pressure relief valves, low-water & over-temp protection.
  • Utilities & Options: steam generator or external boiler, vacuum pump for drying, chart/thermal printer, IQ/OQ/PQ documents.

Applications of Horizontal High Pressure Autoclave

  • Hospitals & CSSD: Sterilization of surgical instruments, wrapped sets, drapes, and linen with validated Class B cycles ensuring patient safety and infection control.
  • Pharmaceutical & Biotech: Sterilizing culture media, glass vials, rubber bung/stoppers, and device components in compliance with cGMP and quality norms.
  • Research & Academia: Reliable sterilization for microbiology, life-science labs, and universities handling culture tubes, petri dishes, and lab ware.
  • Food, Dairy & Veterinary: Decontamination of tools, containers, and process accessories to maintain hygiene and biosafety standards.

Benefits of a Horizontal High Pressure Autoclave

  • Uniform steam penetration and repeatable lethality (F0) across dense and porous loads—validated cycles reduce reprocessing risks.
  • Higher throughput with trolley loading, jacket heating, and fast drying—optimized for bulk hospital and pharma workloads.
  • Compliance-ready with batch records, alarms, and optional printers/data logging to support audits, IQ/OQ/PQ, and GMP documentation.
  • Durable stainless-steel build, advanced interlocks, and efficient insulation delivering long service life and lower operating costs.

Manufacturers & Suppliers in India

India hosts leading autoclave manufacturers serving hospitals, CSSD units, pharma, and research labs. Companies such as DM Sterile Industries (Ahmedabad), along with several established engineering firms across Ahmedabad, Mumbai, Chennai, and Hyderabad, provide horizontal high-pressure autoclaves with PLC controls, pre-vacuum options, and GMP documentation. These suppliers support installation, validation (IQ/OQ/PQ), and lifecycle service to ensure safe, compliant, and efficient sterilization.


Maintenance and Safety Tips

  • Verify door gasket integrity and locking mechanism daily; replace worn gaskets to prevent steam leaks and cycle failures.
  • Descale steam generator/boiler at recommended intervals; use treated water to minimize scaling and sensor drift.
  • Calibrate temperature/pressure sensors periodically; perform Bowie-Dick/Helix tests and biological indicators as per SOPs.
  • Keep chamber and drains clean; remove lint and wrap debris to maintain drainage and uniform steam saturation.
  • Train staff on loading patterns, cycle selection, and PPE; never attempt to open the door under pressure or high temperature.


FAQs – Horizontal High Pressure Autoclave

1. What makes a horizontal high pressure autoclave different from a tabletop unit? +
Horizontal autoclaves have larger jacketed chambers, trolley loading, and higher throughput. They handle bulk wrapped sets, linen, and media with faster heat-up and drying than benchtop units.

2. Which loads are suitable—liquids, instruments, or textiles? +
All three. Programs can be tailored for liquids (slow exhaust), wrapped instruments (pre-vacuum & drying), and textiles/linen, each with specific exposure and exhaust profiles.
3. What temperature and pressure are typically used? +
Common set points are 121°C (~15 psi) and 134°C (~30 psi). Exact parameters depend on the load, packaging, and validation requirements.
4. Do I need a vacuum pump and printer/data logger? +
A vacuum pump improves air removal and drying—recommended for wrapped/porous loads. Printers/data loggers provide batch records for audits and GMP compliance.
5. How do I validate the autoclave performance? +
Conduct IQ/OQ/PQ with thermocouple mapping, Bowie-Dick/Helix tests for air removal, and routine biological indicators to verify sterility assurance levels.


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